A Protocol for Using Förster Resonance Energy Transfer (FRET)-force Biosensors to Measure Mechanical Forces across the Nuclear LINC Complex
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09:43 min
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April 11th, 2017
DOI :
April 11th, 2017
•Rozdziały w tym wideo
0:05
Title
0:53
Transfect Cells with Nesprin-2G and Other Plasmid DNA
4:09
Verify Transfection Efficiency
5:12
Capture Spectral Fingerprints of mTFP1 and Venus Fluorophores for Spectral Unmixing
6:53
Capture Unmixed Images
7:52
Results: Setting Imaging Parameters and Capturing Spectrally Resolved FRET Images
8:51
Conclusion
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