Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy

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Agarose Embedding and Vibratome Slicing of Human Lung Tumors

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Immunostaining of Resident CD8 T Cells and Tumor Cells

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Imaging and Analyzing Resident CD8 T Cell Migration Within Human Lung Tumor Slices

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Results: Distribution and Motility Patterns of Resident CD8 T Cells in Human Lung Tumor Tissues

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Conclusion