Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells
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10:28 min
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January 10th, 2018
DOI :
January 10th, 2018
•1Department of Neurological Surgery, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine, 2Department of Pathology, Johns Hopkins University, School of Medicine, 3Department of Neurological Surgery, University Hospital-Case Medical Center, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine
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0:05
Title
1:17
Generation of Lentiviral Transcriptional Reporter Cell Lines
3:47
Subclone Selection, Expansion, and Validation
4:47
Determination of GFAP:GFP Expression by Flow Cytometry
5:58
ELDA Self-renewal Assay to Assess Clonogenic Capacity
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Drug Screen
8:27
Results: Selection and Drug Screening of Glioblastoma Stem Cell Subclones
9:46
Conclusion
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