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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

11.8K Views

11:51 min

April 27th, 2018

DOI :

10.3791/57017-v

April 27th, 2018


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Selective Pressure Incorporation

Rozdziały w tym wideo

0:05

Title

1:46

Recombinant Protein Expression

4:05

Target Protein Purification via Immobilized Metal Ion Affinity Chromatography

5:20

Intact Protein Mass Analysis by High-performance Liquid Chromatography Coupled to Electrospray Ionization Time-of-flight Mass Spectrometry

6:38

Fluorescence Lifetime Measurements and Decay-associated Spectra of GdFP

7:59

Results: Gold Fluorescent Protein Analysis by Mass Spectrometry and Fluorescence

10:13

Conclusion

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