Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker

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9.7K Views

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13:26 min

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March 31st, 2018

DOI :

10.3791/57153-v

March 31st, 2018

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Jennifer Eom¹, Vaughan Feisst¹, Louis Ranjard², Kerry Loomes¹, Tanvi Damani¹, Victoria Jackson-Patel¹^,³, Michelle Locke⁴^,⁵, Hilary Sheppard¹, Pritika Narayan¹^,⁶, P. Rod Dunbar¹^,⁷

¹School of Biological Sciences, The University of Auckland, ²Research School of Biology, The Australian National University, ³Auckland Cancer Society Research Centre, Faculty of Medical and Health Sciences, The University of Auckland, ⁴Department of Plastic Surgery, Counties Manukau District Health Board, ⁵Department of Surgery, Faculty of Medical and Health Sciences, The University of Auckland, ⁶Biomedical Imaging Research Unit, Faculty of Medical and Health Sciences, The University of Auckland, ⁷Maurice Wilkins Centre, The University of Auckland

Podsumowanie

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Keywords Mesenchymal Cell

Adipogenic Differentiation

Lineage specific Marker

Fatty Acid Binding Protein 4

Stromal Cells

High content Screening

Immunofluorescent Microscopy

Differentiation Medium

Rozdziały w tym wideo

0:00

Title

1:55

Inducing the differentiation of human adipose derived stromal cells

3:36

Staining for differentiation

5:53

Imaging cells using high content screening microscope

8:23

Image Analysis: Cell Scoring

9:43

Image Analysis: Transfluor

10:43

Representative results

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