A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells

Please note that all translations are automatically generated. Click here for the English version.

11.5K Views

•

11:51 min

•

July 5th, 2018

DOI :

10.3791/57361-v

July 5th, 2018

•

Effie E Bastounis¹, Fabian E Ortega¹, Ricardo Serrano², Julie A Theriot³

¹Department of Biochemistry, Stanford University School of Medicine, ²Department of Mechanical and Aerospace Engineering, University of California San Diego, ³Departments of Biochemistry, Microbiology and Immunology and Howard Hughes Medical Institute, Stanford University School of Medicine

Podsumowanie

Przeglądaj więcej filmów

Keywords Polyacrylamide

Host pathogen Biomechanics

Fluorescent Microbeads

Rozdziały w tym wideo

0:05

Title

0:39

Manufacturing Thin Two-layered Polyacrylamide Hydrogels on Multi-well Plates

1:36

Polyacrylamide Hydrogel Fabrication

5:22

Infection of Human Microvascular Endothelial Cells with L. monocytogenes

7:11

Flow Cytometry to Quantify Extracellular-matrix-stiffness Dependent Susceptibility of Host Cells to Infection

8:02

Quantitative Time-lapse Microscopy to Assess Extracellular-matrix-stiffness Dependent L. monocytogenes Dissemination Through Endothelial Cells

9:01

Results: The Effect of Extracellular Matrix Stiffness on L. monocytogenes Infection of HMEC-1 Cells

10:32

Conclusion

Powiązane Filmy

11:31

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens

13.4K Views

13:28

Introducing Shear Stress in the Study of Bacterial Adhesion

15.6K Views

08:45

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

15.5K Views

09:35

Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis

11.6K Views

09:20

Investigating the Effects of Probiotics on Pneumococcal Colonization Using an In Vitro Adherence Assay

12.5K Views

11:30

Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow

10.2K Views

09:25

Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells

10.9K Views

10:55

Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance

8.0K Views

08:09

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

8.0K Views

11:12

Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm

8.4K Views