A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

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10.0K Views

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09:12 min

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June 15th, 2018

DOI :

10.3791/57399-v

June 15th, 2018

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Ola H. Negm¹^,², Mohamed Hamed¹^,², Tanya M. Monaghan³

¹Breast Surgery Group, Division of Medical Sciences and Graduate Entry Medicine, School of Medicine, Queen's Medical Centre, University of Nottingham, ²Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, ³Nottingham Digestive Diseases Centre and NIHR Nottingham Digestive Diseases Biomedical Research Centre, School of Medicine, Queen's Medical Centre, University of Nottingham

Podsumowanie

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Keywords Protein Microarray

Clostridium Difficile

Rozdziały w tym wideo

0:04

Title

1:14

Preparing a Microarray Plate and Printing with a Contact Robot

3:06

Array Probing

5:19

Array Scanning

6:36

Results: Validation of Protein Microarray to Detect Isotype Specific Antibody Response to Clostridium difficile Toxins and Its Reproducibility

8:01

Conclusion

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