JoVE Logo

Zaloguj się

A Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Platform for Investigating Peptide Biosynthetic Enzymes

7.8K Views

11:32 min

May 4th, 2020

DOI :

10.3791/61053-v

May 4th, 2020


Przeglądaj więcej filmów

Hydrogen Deuterium Exchange

Rozdziały w tym wideo

0:04

Introduction

0:36

Bottom-Up Liquid Chromatography-Mass Spectrometry (LC-MS) Reference Sample Workflow Preparation

1:18

Reference Data Processing and Peptide List Definition

5:05

Hydrogen-Deuterium Exchange (HDX) Reactions

6:36

HDX Data Processing, Analysis, and Visualization

9:03

Results: Reapresentative HDX-MS Peptide Biosynthetic Enzyme Evaluation

10:44

Conclusion

Powiązane Filmy

article

11:37

Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry

18.3K Views

article

09:18

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

9.8K Views

article

09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

13.7K Views

article

10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

10.0K Views

article

11:14

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

15.7K Views

article

10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

8.0K Views

article

10:24

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

8.6K Views

article

05:45

Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry

3.2K Views

article

07:15

Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation

17.7K Views

article

08:40

Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions

2.8K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone