In-Nucleus Hi-C in Drosophila Cells
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11:58 min
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September 15th, 2021
DOI :
September 15th, 2021
•Rozdziały w tym wideo
0:05
Introduction
1:13
Schneiders Line 2 Plus (S2R+) Drosophila Cell Fixation
2:02
Lysis
3:18
Enzymatic Digestion, DNA End Biotinylation, and Ligation
4:37
Crosslink Reversal and DNA Purification
5:33
Hi-C Template Quality Evaluation
6:26
Biotin Removal/End Repair and Size Selection
7:49
Biotin Pulldown/A-Tailing/Adapter Ligation
9:33
Results: Representative In-Nucleus Hi-C Analysis
11:17
Conclusion
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