Fluorescence-Activated Nuclei Negative Sorting of Neurons Combined with Single Nuclei RNA Sequencing to Study the Hippocampal Neurogenic Niche

1:08

Dissection of the Dentate Gyrus (DG)

1:53

Tissue Dissociation, Single Nuclei Isolation, and Anti-NeuN Immunostaining

4:03

Fluorescence Activated Nuclei Sorting (FANS) to Exclude Neuronal Populations

5:12

Preparation of the Single Nuclei Suspension to Perform Single Nuclei RNA Sequencing

5:59

Results: Preparation of a Suspension of Non-Neuronal Single Nuclei Isolated from the Dentate Gyrus (DG)

7:51

Conclusion