In the protocol, we describe a feasible method to obtain the cardiac extracellular matrix in vitro as a substrate for studying the cell-matrix interactions and the influence of myocardium-specific extracellular matrix on cardiac cells. Current lines of research focus on development of bioengineered, three-dimensional supports made of biomimetic materials that can be seeds with cells. This material seem to provide biomechanical and spatial cues for cells.
The composition of extracellular matrix has a profound impact on cells, with the bi-directional signaling taking place and leading to continuous modifications in both matrix composition and cell behavior. In vitro models reproducing cardiac microenvironment, typical of physiological or pathological conditions are missing. A better understanding of cell matrix interactions offered by the ability to recreate them in vitro can significantly improve the design of biomimetic scaffolds for tissue engineering and regeneration.
