Determination of Sialic Acids in Liver and Milk Samples of Wild-type and <em>CMAH</em> Knock-out Mice.

CMAH (cytidine monophosphate-N-acetylneuraminic acid hydroxylase) is responsible for the oxidation of cytidine monophosphate-N-acetylneuraminic acids in ...

Nanjing Agricultural University

A five-step assembly of silicon-protected dipeptide mimics from commercially available reagents is described. This methodology makes silanediol protease inhibitors readily available for the first time. The sequence features asymmetric hydrosilylation, a novel reduction of a silyl ether to a silyllithium reagent, and addition of this dianion to a sulfinimine, to produce the complete inhibitor skeleton with full control of stereochemistry. Oxidation of the primary alcohol to an acid completes the synthesis.

Efficient, enantioselective assembly of silanediol protease inhibitors.

Parinaric acid methyl ester (PnA-Me) polymer films with hill-structured features were fabricated by a solvent volatilization in situ cross-linking method. Moreover, nuclear magnetic resonance, Fourier transform infrared, and oxidation kinetic analyses were successfully applied to monitor the formation process of PnA-Me polymer films. The role of PnA-Me monomer concentrations for growth control of the hill structures on a glass matrix had also been investigated. Also, the results demonstrated that size control of the resulting hill structure ranging from 0.56 ± 0.18 to 19.6 ± 3.5 μm could be realized by varying the concentration of the PnA-Me monomer from 0.0117 to 1.5 mg/mL. Additionally, the effects of polymer films with different surface topographical structures on the behaviors of rat mesenchymal stem cells and human pheochromocytoma cells were measured by morphological and metabolic methods. The results revealed that the cell activity of PnA-Me films was topographical structure- and cell-type-dependent. Furthermore, the selective sensitivity of the PnA-Me films to normal and tumor cells supported the potential value as the coatings for the tissue engineering substitutes.

Parinaric acid methyl ester polymer films with hill-structured features: fabrication and different sensitivities to normal and tumor cells.

Development of MOF-based photocatalysts is intriguing research due to their structural flexibility and tremendous catalytic sites, whereas most MOFs only can take use of UV/visible light and lack of response to NIR light. Herein, we present a facile approach to integrate upconversion nanoparticles (UCNPs) with MOF to build a NIR-responsive composite photocatalyst. The MOF shell with controllable thickness can be grown on the UCNPs, thus exhibiting tunable photocatalytic activities under NIR irradiation. Furthermore, we extend visible absorption of the MOF shell by adding -NH2 groups so that the composite photocatalysts have a better utilization of UC emissions and sunlight to improve their activities. The developed composite photocatalysts have been characterized by XRD, TEM, PL, etc., and their photocatalytic performances were systematically explored. The formation and working mechanism of the composite photocatalysts were also elucidated.

Controlled Growth of Metal-Organic Framework on Upconversion Nanocrystals for NIR-Enhanced Photocatalysis.

Combination of upconversion nanocrystals (UCNs) with CeO2 is a decent choice to construct NIR-activated photocatalysts for utilizing the NIR light in the solar spectrum. Herein we present a facile approach to deposit a CeO2 layer with controllable thickness on the plate-shaped NaYF4:Yb,Tm UCNs. The developed core-shell nanocomposites display obvious photocatalytic activity under the NIR light and exhibit enhanced activity under the full solar spectrum. For enhancing the separation of photogenerated electrons and holes on the CeO2 surface, we sequentially coat a ZnO shell on the nanocomposites so as to form a heterojunction structure for achieving a better activity. The developed hybrid photocatalysts have been characterized with TEM, SEM, PL, etc., and the working mechanism of such UCN-semiconductor heterojunction photocatalysts has been proposed.

Heterogeneous Semiconductor Shells Sequentially Coated on Upconversion Nanoplates for NIR-Light Enhanced Photocatalysis.

Acute venous thrombosis is prevalent and potentially fatal. Accurate diagnosis of early thrombus is needed for patients in timely clinical intervention to prevent life-threatening conditions. Photoacoustic imaging (PAI) with excellent spatial resolution and high optical contrast shows more promise for this purpose. However, its application is dramatically limited by its signal-off effect on thrombus because of the ischemia in thrombus which lacks the endogenous photoacoustic (PA) signal of hemoglobin. To address this dilemma, we herein report the feasibility of using organic semiconducting nanoparticles (NPs) for contrast-enhanced PAI of thrombus in living mice. An organic semiconducting NP, self-assembled by amphiphilic perylene-3,4,9,10-tetracarboxylic diimide (PDI) molecules, is chemically modified with cyclic Arg-Gly-Asp (cRGD) peptides as a PA contrast agent (cRGD-PDI NPs) for selectively lightening early thrombus. cRGD-PDI NPs presents high PA intensity, good stability in light and serum, and sufficient blood-circulating half-life. In living mice, PA intensity of early thrombus significantly increases after tail vein injection of cRGD-PDI NPs, which is 4-fold greater than that of the control, blocking, and old thrombus groups. Pathological and immunohistochemical findings show that glycoprotein IIb/IIIa abundant in early thrombus is a good biomarker targeted by cRGD-PDI NPs for distinguishing early thrombus from old thrombus by PAI. Such a lightening PAI effect by cRGD-PDI NPs successfully provides accurate information including the profile, size and conformation, and spatial distribution of early thrombus, which may timely monitor the obstructive degree of thrombus in blood vessels and the thrombolysis effect.

Organic Semiconducting Nanoparticles as Efficient Photoacoustic Agents for Lightening Early Thrombus and Monitoring Thrombolysis in Living Mice.

CXCL5, a CXC-type chemokine, is an important attractant for granulocytic immune cells by binding to its receptor CXCR2. Recently, CXCL5/CXCR2 has been found to play an oncogenic role in many human cancers. However, the exact role of CXCL5 in osteosarcoma cell migration and invasion has not been revealed. Here we found that the protein expression of CXCL5 was significantly increased in osteosarcoma tissues compared with that in matched adjacent nontumor tissues. Moreover, the expression of CXCL5 was significantly associated with advanced clinical stage and metastasis. Further investigation showed that the CXCL5 expression levels were also significantly increased in osteosarcoma cell lines, including Saos-2, MG63, U2OS, and SW1353, when compared with those in normal osteoblast hFoB1.19 cells. U2OS cells were further transfected with CXCL5-specific siRNA or overexpression plasmid. Knockdown of CXCL5 significantly suppressed U2OS cell migration and invasion. On the contrary, overexpression of CXLC5 remarkably promoted the migration and invasion of U2OS cells. Interestingly, both exogenous CXCL5 treatment and the conditioned medium of CXCL5-overexpressing hFoB1.19 cells could also enhance the migration and invasion of U2OS cells, suggesting that the promoting role of CXCL5 in U2OS cell migration and invasion is also in a paracrine-dependent manner. According to these data, our study demonstrates that CXCL5 is upregulated in osteosarcoma and may play an oncogenic role in osteosarcoma metastasis. Therefore, CXCL5 may become a potential therapeutic target for osteosarcoma treatment.

Cui Cao

Glycomics and Glycan Bioengineering Research Center (GGBRC),
College of Food Science and Technology,
Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology

Nanjing Agricultural University

Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.

Cui Cao

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Nanjing Agricultural University

Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.

Glycomics and Glycan Bioengineering Research Center (GGBRC),
College of Food Science and Technology,
Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology