This study was supported by the National Natural Science Foundation of China (Nos. 11674344 and 22201026) and the Key Research Program of Frontier Sciences, CAS (Grant NO. QYZDJ-SSW-SLH019).

The details of the plasmids, reagents, and equipment used in this study are listed in the Table of Materials.
1. pH response method
<ol>
	<li>Add 5 mg of ECF-5 peptides to 400 &#181;L of deionized water. Sonicate at 40 kHz for 30 min and mix thoroughly.</li>
	<li>Add 40 &#181;L of sodium hydroxide (1 M, filtered through a 0.22 &#181;m filter) to the peptide solution. Vortex and mix thoroughly. Continue sonication for 15 min until the solution is completely c...

 Peptide Hydrogels: Methods for Environmentally Responsive Gelation of ECF-5

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The authors have no conflicts of interest to disclose.

In the past few decades, following the discovery of self-assembling peptide sequences derived from amyloid proteins, numerous self-assembling peptides have been designed based on their properties, demonstrating significant potential for applications in biomedicine and materials science19. Peptide hydrogels have exhibited unique bio-functionalization capabilities in tissue culture, drug delivery, and tumor treatment20.
This article describes simpl...

Through the design of peptide sequences, non-covalent interactions between peptides induce self-assembly, leading to the formation of ordered micro- and nanometer structures, including nanotubes, nanoribbons, nanofibers, and spherical structures1. When self-assembled into micro- and nanometer fibers/ribbons, these structures macroscopically exhibit hydrogel properties. Peptide self-assembling hydrogels differ from polymer hydrogels in that they self-assemble through non-covalent interactions, their gel form is reversible, and they readily respond to specific conditions to transition between solution and gel phases2. For instance, aromatic amino acid peptides can be induced to gelatinize based on solvent switching3,4,5, RADA16 peptides form gels through cationic and anionic electrostatic interactions6, and E1Y9 peptide is induced to form a hydrogel via Ca2+ ions7. Natural amino acids can be metabolized by the human body and offer excellent biocompatibility, a feature that polymer hydrogels cannot achieve8. Proteins are the molecules that execute biological functions, and differences in peptide sequences create their specific biological functions. Therefore, embedding specific biofunctional peptide sequences and endowing them with self-assembling properties can design peptide self-assembling hydrogels with unique biological functions and morphologies9,10,11. This article introduces three methods for preparing peptide hydrogels, where the gelation process is triggered by environmental responsiveness. It also briefly discusses methods for characterizing the mechanical properties and morphology of peptide hydrogels.
The pH regulates the charge of amino acids, triggering the gelation of some peptides. For instance, positively charged amino acids (e.g., arginine, lysine, histidine) are regulated by pH to attain positive or neutral states. Negatively charged amino acids are regulated by pH to achieve negative or neutral states, moving away from their isoelectric point and thereby altering their hydrophilicity in aqueous solutions. Therefore, controlling electrostatic and hydrophobic interactions between peptides facilitates their ordered self-assembly. Zhang et al. designed an amphiphilic pH-responsive self-assembling peptide, methotrexate-coupled KKFKFEFEF, which responds to slightly acidic environments both in vitro and in vivo, enabling a sol-to-gel phase transition. This leads to efficient cellular uptake and endocytosis, thereby delivering anti-cancer drugs and improving chemotherapy effectiveness12. Shen et al.13 designed the FF8 (KRRFFRRK) peptide, which easily self-assembles into fibers at a pH greater than 9.4. Under neutral conditions, microorganisms neutralize their positive charges due to electrostatic interactions with their negatively charged phospholipid membranes, coordinating with phospholipid molecules to self-assemble, causing membrane rupture and enhancing bactericidal effects13.
Triggering peptide supramolecular self-assembly into hydrogels using coordination metals is a relatively rare method14. When metal ions interact electrostatically with peptides, they form salt bridges that connect peptide molecules, leading to non-covalent interactions and self-assembly, which results in gelation properties. For example, Abul-Haija et al.15 designed the tripeptide FFD, which transitions from a liquid to a hydrogel upon the addition of copper ions. Tao et al.16 developed the glutamic acid and phenylalanine-rich peptide E3F3, which self-assembles into fibrous hydrogels in the presence of zinc ions, and is used for prostate drug delivery.
Solvent exchange formation of peptide hydrogels is the most common supramolecular self-assembly triggering condition. After hydrophobic peptides dissolve in organic solvents, their hydrophobic groups are fully exposed. When transferred to an aqueous phase, the hydrophobic groups approach each other, and water molecules facilitate the formation of peptide hydrogen bonds, leading to rapid self-assembly and easy formation of hydrogels. For instance, Zhang et al.17 designed a peptide that could dissolve stably at high concentrations in polar organic solvents and, upon dilution with water, self-assembled into &#946;-sheet structures to form peptide fiber hydrogels. Shen et al.13 designed a reductive peptide ECF-5 (ECAFF), pre-dissolved in dimethyl sulfoxide (DMSO) and then injected into an aqueous phase to form a reductive hydrogel, used for the targeted removal of reactive oxygen species produced by ischemia-reperfusion, which subsequently degraded into a solution after scavenging.
This study selected three simple, rapid, and highly generalizable peptide hydrogel preparation strategies based on previous experiences: (1) pH response method: peptides are dissolved in a solution with a pH far from their isoelectric point, and then the pH is adjusted to near the isoelectric point. This change allows certain self-assembling peptides to form fibers and create peptide hydrogels; (2) Metal ion addition method: coordination cations are added to water-soluble, negatively charged self-assembling peptides. The metal coordination chelation between peptides leads to their self-assembly into hydrogels; (3) Solvent exchange method: high-concentration peptides are dissolved in organic solvents and then diluted into an aqueous phase, inducing gelation behavior.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr >
			<td >3-Aminopropyl)triethoxysilane</td>
			<td >Aladdin</td>
			<td >A107147</td>
			<td >/</td>
		</tr>
		<tr >
			<td >Atomic Force Microscopy</td>
			<td>Bruker</td>
			<td>Multimode Nanoscope VIII</td>
			<td>/</td>
		</tr>
		<tr >
			<td >CaCl2</td>
			<td>Aladdin</td>
			<td>C290953</td>
			<td>/</td>
		</tr>
		<tr >
			<td >Diphenylalanine (FF)</td>
			<td>Chinesepeptide</td>
			<td>customizable</td>
			<td>Purity &#62; 95%</td>
		</tr>
		<tr >
			<td >DMSO</td>
			<td>Sigma-aldrich</td>
			<td>34869</td>
			<td>/</td>
		</tr>
		<tr >
			<td >ECF-5 Peptides</td>
			<td>Chinesepeptide</td>
			<td>sequence: ECAFF</td>
			<td>Purity &#62; 95%</td>
		</tr>
		<tr >
			<td >Hydrochloric Acid</td>
			<td>Aladdin</td>
			<td>H399657&#160;</td>
			<td>/</td>
		</tr>
		<tr >
			<td >Mica</td>
			<td>Sigma-aldrich</td>
			<td>AFM-71856-02</td>
			<td>/</td>
		</tr>
		<tr >
			<td >Phosphate Buffered Saline</td>
			<td>Aladdin</td>
			<td>P492453</td>
			<td>/</td>
		</tr>
		<tr >
			<td >Rheometer</td>
			<td>Anton Paar GmbH</td>
			<td>MCR302</td>
			<td>/</td>
		</tr>
		<tr >
			<td >Silicon Cantilevers</td>
			<td>MikroMasch</td>
			<td>XSC11</td>
			<td>/</td>
		</tr>
		<tr >
			<td >Sodium Chloride</td>
			<td>Aladdin</td>
			<td>C111549</td>
			<td>/</td>
		</tr>
		<tr >
			<td >Sodium Hydroxide</td>
			<td>Aladdin</td>
			<td>S140903</td>
			<td>/</td>
		</tr>
		<tr >
			<td >TRIS Hydrochloride</td>
			<td>Aladdin</td>
			<td>T431531</td>
			<td>/</td>
		</tr>
	</tbody>
</table>

preparation of mechanically stable self-assembled peptides hydrogels

Peptide hydrogels are highly hydrophilic, three-dimensional network gels formed by the self-assembly of nanofibers or polymers, creating water-locking networks. Their morphology closely resembles that of the extracellular matrix, allowing them to exhibit both the biological functions of peptides and responsive gelation properties. These unique characteristics have led to their extensive application in tissue engineering, three-dimensional cell culture, cancer therapy, regenerative medicine, and other biomedical fields. This article describes three methods for preparing ECF-5 peptide hydrogels using self-assembling peptides with environmentally responsive gelation processes: (1) pH-responsive gelation: varying pH levels induce the protonation or deprotonation of amino acid residues, altering electrostatic interactions between peptide molecules and promoting their self-assembly into hydrogels; (2) Metal ion addition: polyvalent metal ions chelate with negatively charged amino acid residues, acting as bridges between peptides to form a network hydrogel; (3) Solvent exchange: hydrophobic peptides are initially dissolved in non-polar organic solvents and subsequently induce self-assembly into hydrogels upon transitioning to a polar aqueous environment. These methods utilize conventional experimental procedures to facilitate peptide self-assembly into hydrogels. By designing peptide sequences to align with specific gelation-inducing conditions, it is possible to achieve finely tuned micro/nanostructures and biological functions, highlighting the significant potential of peptide hydrogels in the biomedical domain.

Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility

Preparation of Mechanically Stable Self-Assembled Peptides Hydrogels

My research, exploring peptide functionalization, focusing on self-assembly biofunctionalized fibers, hydrogels, and responsive separations. This video describe how ECF-5 peptides self-assembly in response to environmental changes and the process of generating peptide hydrogels. Compared to traditional polymer hydrogels, peptide hydrogels offer diversified biofunctions and superior biocompatibility, enhancing their potential in field like biomedical engineering and biomaterials.Technological breakthroughs in this field focused on improving self-assembly performance and biofunctionalization. Our reagents include peptide hydrogels with non-natural amino acid, faster point chemical medication, and lutein-specific biofunctionalized peptides, thereby enhance their gel forming and biofunctional properties. Current peptide gel forming method have high experimental demands and a lack of universality.Developing self-assembled hydrogel formation methods using common operations and reagents will better support the widespread application of peptide hydrogels. Using the ECF-5 peptide, we added phase three generalized and environmentally-responsible organization methods. This methods rotating the peptide surfaces properties.We are aiding in the design other self-assembling peptide with the improved organization control.

The three methods described in this article for preparing peptide hydrogels enable rapid, affordable, and straightforward production. The function of the hydrogel is related to its peptide sequence. Here, the ECF-5 peptide is used as a representative example to demonstrate its physical characteristics, including microscopic morphology and mechanical properties.
As shown in Figure 1A and Supplementary Figure 1, the ECF-5 peptide contains a glutathi...

This protocol presents three fast and simple preparation methods that use environmental conditions to trigger the self-assembly of peptides into hydrogels. Additionally, the characterization of peptide hydrogels is described, demonstrating that mechanically stable peptide hydrogels can be formed under these straightforward conditions.

Peptide hydrogels are highly hydrophilic, three-dimensional network gels formed by the self-assembly of nanofibers or polymers, creating water-locking ...

preparation-of-mechanically-stable-self-assembled-peptides-hydrogels

Research

JoVE Journal

Biochemistry

Preparation of ECF-5 Peptide Hydrogels for Regenerative Medicine Using pH Response Method

To begin the pH response method for hydrogel formation, add five milligrams of ECF-5 peptides to 400 microliters of deionized water, and sonicate it at 40 kilohertz for 30 minutes. Add 40 microliters of filtered one-molar sodium hydroxide to the peptide solution, and vortex to mix thoroughly. Continue sonication for 15 more minutes until the solution is completely clarified.Next, add 60 microliters of hydrochloric acid to the prepared solution. After vortexing the mixture, let it stand at room temperature for over 30 minutes to facilitate hydrogel formation.

Metal Ion Addition and Solvent Exchange Approaches to ECF-5 Peptide Hydrogel Formation for Enhanced Biomedical Applications

To begin, prepare an ECF-5 self-assembling peptide solution at a concentration of 10 milligrams per milliliter using a suitable buffer. Sonicate the mixture to dissolve the peptide, and store it at four degrees Celsius. Prepare a 50 milligrams per milliliter calcium chloride solution using deionized water.Add 40 microliters of calcium chloride solution to 460 microliters of the ECF-5 peptide solution. Vortex to mix thoroughly, and incubate the mixture at room temperature for over two hours. Rapidly introduce one milliliter of PBS into the dimethyl sulfoxide or DMSO solubilized peptide.Vortex thoroughly and incubate at room temperature. After five minutes, add 500 microliters of PBS, and let the mixture stand for 15 minutes. Then discard the supernatant and retain the bottom gel.The ECF-5 peptide displayed glutathione-like reducing properties and self-assembly capability, forming a solid gel within minutes when the concentration exceeded 1%The ECF-5 hydrogel exhibited semi-solid properties when prepared via pH response, metal edition, or solvent exchange.

Atomic Force Microscopy to Characterize the Fiber Morphology of ECF-5 Peptide Hydrogels for Tissue Engineering

To begin, prepare the peptide hydrogels using a suitable method. Apply double-sided adhesive to remove a layer of mica, and adhere the mica surfaces using the APTES solution and let it stand for five minutes. Rinse the mica surfaces thoroughly with pure water before use.Dilute the hydrogel and mix it thoroughly before dropping it onto the modified mica surface for static absorption. After five minutes, rinse the surface with pure water and dry it. Atomic force microscopy revealed that the gel surface had numerous peptide fibers with dense pores trapping water, with individual fibers forming consistent wider fiber bands, approximately 3.5 nanometers in height.

434 Views.  The First Affiliated Hospital of Nanchang University.  This protocol presents three fast and simple preparation methods that use environmental conditions to trigger the self-assembly of peptides into hydrogels. Additionally, the characterization of peptide hydrogels is described, demonstrating that mechanically stable peptide hydrogels can be formed under these straightforward conditions.

Peptide Hydrogels: Methods for Environmentally Responsive Gelation of ECF-5

Summary