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United States Army Institute of Surgical Research

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres

David O. Zamora^*1, Shanmugasundaram Natesan^*1, Robert J. Christy¹

¹Department of Regenerative Medicine, United States Army Institute of Surgical Research

A major hurdle in current stem cell therapies is determining the most effective method to deliver these cells to host tissues. Here, we describe a chitosan-based delivery method that is efficient and simple in approach, while allowing adipose-derived stem cells to maintain their multipotency.

Bioengineering

Engineering a Bilayered Hydrogel to Control ASC Differentiation

Shanmugasundaram Natesan¹, David O. Zamora¹, Laura J. Suggs², Robert J. Christy¹

¹Department of Extremity Trauma Research and Regenerative Medicine, United States Army Institute of Surgical Research, ²Department of Biomedical Engineering, The University of Texas at Austin

This protocol focuses on utilizing the inherent ability of stem cells to take cue from their surrounding extracellular matrix and be induced to differentiate into multiple phenotypes. This methods manuscript extends our description and characterization of a model utilizing a bilayered hydrogel, composed of PEG-fibrin and collagen, to simultaneously co-differentiate adipose-derived stem cells¹.