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Showa University

3 ARTICLES PUBLISHED IN JoVE

Medicine

2-Methacryloyloxyethyl Phosphorylcholine Polymer Treatment of Complete Dentures to Inhibit Denture Plaque Deposition

Kenji Ikeya¹, Miya Fukunishi¹, Fuminori Iwasa¹, Yuuki Inoue², Kazuhiko Ishihara², Kazuyoshi Baba¹

¹Department of Prosthodontics, School of Dentistry, Showa University, ²Department of Materials Engineering, School of Engineering, The University of Tokyo

Removable poly(methyl methacrylate) (PMMA) dentures are prone to bacterial adherence and plaque formation. Denture plaque-associated infection is a source of serious dental and medical complications in the elderly. This paper introduces a novel protocol to treat PMMA dentures with 2-methacryloyloxyethyl phosphorylcholine polymer, poly(MPC-co-BMA-co-MPAz), to suppress plaque deposition on PMMA dentures.

Cancer Research

Establishing Dual Resistance to EGFR-TKI and MET-TKI in Lung Adenocarcinoma Cells In Vitro with a 2-step Dose-escalation Procedure

Toshimitsu Yamaoka¹, Motoi Ohba¹, Satoru Arata^1,2, Tohru Ohmori¹

¹Institute of Molecular Oncology, Showa University, ²Center for Biotechnology, Showa University

An in vitro method for establishing dual resistance to an EGFR-TKI and a MET-TKI in cancer cells is described. This method is useful for developing treatments for patients with EGFR-mutations, who exhibit disease progression despite EGFR-TKI treatment with MET-amplification. It can also be modified for inhibitors targeting other molecules.

Cancer Research

Establishment and Characterization of Three Afatinib-resistant Lung Adenocarcinoma PC-9 Cell Lines Developed with Increasing Doses of Afatinib

Toshimitsu Yamaoka¹, Motoi Ohba¹, Yuki Matsunaga², Junji Tsurutani¹, Tohru Ohmori³

¹Advanced Cancer Translational Research Institute, Showa University, ²Division of Breast Surgical Oncology, Department of Surgery, Showa University School of Medicine, ³Division of Allergology and Respiratory Medicine, Department of Medicine, Showa University School of Medicine

A method for establishing afatinib-resistance cell lines from lung adenocarcinoma PC-9 cells was developed, and resistant cells were characterized. The resistant cells can be used to investigate epidermal growth factor receptor tyrosine kinase inhibitor-resistance mechanisms, applicable for patients with non-small cell lung cancer.