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National Defense Medical College

3 ARTICLES PUBLISHED IN JoVE

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Medicine

Microscopic Observation of Lymphocyte Dynamics in Rat Peyer's Patches
Kazuhiko Shirakabe 1,2, Masaaki Higashiyama 1, Naoki Shibuya 2, Kazuki Horiuchi 1, Masayuki Saruta 2, Ryota Hokari 1
1Department of internal medicine, National Defense Medical College, 2Division of Gastroenterology and Hepatology, Department of Internal Medicine, Jikei University School of Medicine

Here, we describe a precise method for collecting thoracic duct lymphocytes and observing the migration of gut-tropic lymphocytes in rat Peyer’s patches for 3 hours using time-lapse photography. This technique can clarify how the dynamics of lymphocytes are affected under inflammatory conditions.

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Medicine

Research Application of Laser-Induced Shock Wave for Studying Blast-Induced Cochlear Injury
Takaomi Kurioka 1, Kunio Mizutari 1, Katsuki Niwa 1, Eiko Kimura 1, Satoko Kawauchi 2, Yasushi Kobayashi 3, Shunichi Sato 2
1Department of Otolaryngology, Head and Neck Surgery, National Defense Medical College, 2Division of Bioinformation and Therapeutic Systems, National Defense Medical College, 3Department of Biochemistry, National Defense Medical College

Here, we describe experimental protocols for creating an animal model of blast-induced cochlear injury using laser-induced shock wave (LISW). Exposure of the temporal bone to LISW allows the reproduction of blast-induced cochlear pathophysiology. This animal model could be a platform for elucidating cochlear pathology and exploring potential treatments for blast injuries.

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Neuroscience

Evaluation of Blood-Brain Barrier Breakdown in a Mouse Model of Mild Traumatic Brain Injury
Kiyomasa Nishii 1, Takahito Higashi 1, Toshiaki Ishizuka 2, Yasushi Satoh 3, Yasushi Kobayashi 1
1Department of Anatomy and Neurobiology, National Defense Medical College, 2Department of Pharmacology, National Defense Medical College, 3Department of Biochemistry, National Defense Medical College

A method was developed to visualize dye extravasation due to blood-brain barrier (BBB) breakdown by administering two fluorescent dyes to mice at different time points. The use of glycerol as a cryoprotectant facilitated immunohistochemistry on the same sample.

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