Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal CancerIrene Chicote 1, Jordi Martínez-Quintanilla 1, Juan Antonio Cámara 2, Héctor G. Palmer 1,3
1Stem Cells and Cancer Laboratory, Vall d’Hebron Institute of Oncology (VHIO), 2Preclinical Therapeutics Core, University of California San Francisco, 3CIBERONC
This protocol describes the orthotopic implantation of patient-derived cancer cells in the cecum wall of immunodeficient mice. The model recapitulates advanced colorectal cancer metastatic disease and allows for the evaluation of new therapeutic drugs in a clinically relevant scenario of lung and liver metastases.
