Environmental and Occupational Health Sciences Institute

2 ARTICLES PUBLISHED IN JoVE

Biology

Whole-Body Nanoparticle Aerosol Inhalation Exposures

Jinghai Yi^1,2, Bean T. Chen³, Diane Schwegler-Berry³, Dave Frazer³, Vince Castranova³, Carroll McBride¹, Travis L. Knuckles^1,2, Phoebe A. Stapleton^1,2, Valerie C. Minarchick^1,2, Timothy R. Nurkiewicz^1,2

¹Department of Physiology and Pharmacology, School of Medicine, West Virginia University , ²Center for Cardiovascular and Respiratory Sciences, West Virginia University , ³National Institute for Occupational Safety and Health

A whole-body nanoparticle aerosol inhalation exposure facility was constructed for nano-sized titanium dioxide (TiO₂) inhalation toxicology studies. This system provides nano-TiO₂ aerosol test atmospheres that have: 1) a steady mass concentration; 2) a homogenous composition free of contaminants; and 3) a stable particle size distribution during aerosol generation.

Developmental Biology

Ex Vivo Perfusion of the Rodent Placenta

Jeanine N. D'Errico¹, Sara B. Fournier², Phoebe A. Stapleton^1,2

¹Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy, Rutgers University, ²Environmental and Occupational Health Sciences Institute

Here is presented a protocol of ex vivo maternal-fetal vascular perfusion to enable the administration of a test article into maternal vasculature and to evaluate placental transfer of xenobiotic particles or pharmacological agents in addition to alterations in placental physiology.