A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned MediaJi-Hye Jung 1, Byung Soo Kim 1
1Department of Biomedical Science, Graduate School of Medicine, Korea University
This protocol provides a simple and efficient way to propagate human pluripotent stem cells (hPSCs) using only conditioned media derived from the human placenta in a gelatin-coated dish without additional exogenous supplementation or hPSC-specific synthetic substrata.