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Hangzhou Medical College

5 ARTICLES PUBLISHED IN JoVE

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JoVE Journal

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
Jing Guo 1, Sifan You 1, Zhichang Wang 1, Jinbo Peng 1, Runze Ma 1, Ying Jiang 1,2
1International Center for Quantum Materials, School of Physics, Peking University, 2Collaborative Innovation Center of Quantum Matter

Here, we present a protocol to investigate the structure and dynamics of interfacial water at the atomic scale, in terms of submolecular resolution imaging, molecular manipulation, and single-bond vibrational spectroscopy.

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Bioengineering

Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632
Zhiwei Xie 1,2,3, Jizhou Shi 4, Min Zong 3, Qiuping Xu 1, Chang Liu 1, Jie Wen 1, Qun Zhang 1, Panpan Liu 1, Guanyi Liu 1, Jing Guo 2,5, Xunwei Wu 1
1Department of Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 2Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 3Department of Stomatology, Shengli Oilfield Central Hospital, 4Department of Pediatric Surgery, Shengli Oilfield Central Hospital, 5Ningbo Stomatology Hospital of Savaid Stomatology School, Hangzhou Medical College

Here we present a modified method for the isolation and culture of human gingival epithelial cells by adding the Rock inhibitor, Y-27632, to the traditional method. This method is easier, less time-consuming, enhances stem cell properties, and produces larger numbers of high-potential epithelial cells both for the laboratory and for clinical applications.

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Neuroscience

Ultrasound-Guided Needle Release Combined with Corticosteroid Injection for the Treatment of Supinator Syndrome
Zeng Zeng 1,2, Cong-Xian Chen 1
1Cancer Center, Department of Ultrasound Medicine, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital, Hangzhou Medical College, 2Zhejiang University School of Medicine

The deep branch of the radial nerve can easily be compressed at the arcade of Frohse due to its anatomical features. Ultrasound-guided needle release combined with corticosteroid injection is an effective and safe treatment for deep branch radial nerve adhesion.

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Biology

Hand-Rearing Method for Infant Marmosets
Hao Sun 1,2, Rui Li 1, Yingxu Lin 1,2, Xinyuan Cao 1, Lingzhu Fan 1, Guanglong Sun 1, Min Xie 1, Lin Zhu 1, Chencen Yu 1, Ruolan Cai 1, Chenfei Lyu 1, Xiaohui Wang 1,2, Yuanqing Zhang 1,2, Siyi Bai 1,2, Runze Qi 1, Binliang Tang 1,3, Guoqiang Jia 1,4, Xinjian Li 1,2,4, Lixia Gao 1,2,4
1Department of Neurology of the Second Affiliated Hospital and Interdisciplinary Institute of Neuroscience and Technology, School of Medicine, Zhejiang University, 2Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, 3Rehabilitation Medicine Center, Rehabilitation & Sports Medicine Research Institute of Zhejiang Province, Department of Rehabilitation Medicine, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital, Hangzhou Medical College, 4NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Science and Brain-Machine Integration, School of Brain Science and Brain Medicine, Zhejiang University

Here, we describe a hand-rearing method for raising infant marmosets in an animal incubator. This method greatly increases the survival rate of marmoset infants, which provides the opportunity to study the development of marmoset infants with similar genetic backgrounds raised in different postnatal environments.

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Biology

Isolation and Culture of Primary Human Mammary Epithelial Cells
Linfeng Qian *1, Jiaxi Yan *1, Shiqi Chen 1, Maryam Mohanmmed Abbas Karekad 2, Yue Wu 3,4, Xunwei Wu 5, Xiaohong Xu 3,4, Xiaoqun Zhang 6,7
1The First Clinical Medical College of Zhejiang Chinese Medical University, 2The International Education College of Zhejiang Chinese Medical University, 3Department of Breast Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), 4Bozhou District Hospital of Traditional Chinese Medicine, 5Engineering Laboratory for Biomaterials and Tissue Regeneration, Ningbo Stomatology Hospital, Ningbo, China and Savaid Stomatology School, Hangzhou Medical College, 6Department of Surgery, Kaihua District, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), 7Kaihua County Hospital of Traditional Chinese Medicine

The present study reports an easier, time-saving, and economical protocol to efficiently isolate and grow primary human mammary epithelial cells (HMECs) from small amounts of mammary tissue. This protocol is suitable for quickly producing primary HMECs both for laboratory and clinical applications.

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