Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity TagJohan Nilvebrant 1, Tove Alm 1, Sophia Hober 1
1School of Biotechnology, Department of Proteomics, Royal Institute of Technology
A novel and highly efficient two-step affinity chromatography protocol has been developed and is described in detail. The method is based on a small purification tag with two inherent affinities and is applicable to a wide range of target proteins with different properties.
