Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide ArraysSrikanth Kudithipudi 1, Denis Kusevic 1, Sara Weirich 1, Albert Jeltsch 1
1Institute of Biochemistry, Stuttgart University
Peptide arrays synthesized by the SPOT method can be used to analyze the substrate specificity of Protein lysine methyltransferases (PKMTs) and to define the substrate spectrum of PKMTs to understand their biological role. This protocol describes how to synthesize peptide arrays, methylate them with PKMTs, and analyze the results.
