Universidade de Sao Paulo

5 ARTICLES PUBLISHED IN JoVE

Neuroscience

Positron Emission Tomography Imaging for In Vivo Measuring of Myelin Content in the Lysolecithin Rat Model of Multiple Sclerosis

Daniele de Paula Faria¹, Caroline Cristiano Real¹, Larissa Estessi de Souza¹, Alexandre Teles Garcez², Fabio Luis Navarro Marques¹, Carlos Alberto Buchpiguel¹

¹Laboratory of Nuclear Medicine (LIM-43), Departamento de Radiologia e Oncologia, Faculdade de Medicina, Universidade de Sao Paulo, ²Nuclear Medicine Division, Hospital Sirio-Libanes

This protocol has the aim of monitoring in vivo myelin changes (demyelination and remyelination) by positron emission tomography (PET) imaging in an animal model of multiple sclerosis.

Neuroscience

Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings

Josiane do Nascimento Silva¹, Thais T. Zampieri¹, Henrique R. Vieira¹, Renata Frazao¹

¹Department of Anatomy, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo

Here, we present a protocol to perform a whole-cell patch-clamp on brain slices containing kisspeptin neurons, the primary modulator of gonadotrophin-releasing hormone (GnRH) cells. By adding knowledge about kisspeptin neuron activity, this electrophysiological tool has served as the basis for significant advancements in the neuroendocrinology field over the last 20 years.

Medicine

Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch

Thaís Girão-Silva^*1,2, Miriam Helena Fonseca-Alaniz^*1, Luís Alberto Oliveira Dallan¹, Iuri Cordeiro Valãdao¹, Gustavo Henrique Oliveira da Rocha¹, José Eduardo Krieger¹, Ayumi Aurea Miyakawa¹

¹Instituto do Coraçao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, ²Cardiology Research Group, Faculty VI Medicine and Health Sciences, Carl von Ossietzky University of Oldenburg

We describe a protocol to isolate and culture human saphenous vein endothelial cells (hSVECs). We also provide detailed methods to produce shear stress and stretch to study mechanical stress in hSVECs.

Medicine

Establishment of an Ex Vivo Lung Perfusion Rat Model for Translational Insights in Lung Transplantation

Paolo Oliveira^*1,3, Keiji Yamanashi^*1, Aizhou Wang¹, Marcelo Cypel^1,2

¹Toronto General Hospital Research Institute, University Health Network, ²Department of Surgery, University of Toronto, ³Departamento de Cardiopneumologia, Instituto do Coração, Faculdade de Medicina HCFMUSP, Universidade de Sao Paulo

Here, we describe the necessary steps for establishing a rat EVLP model and show the inflammatory profile associated with the perfused lungs. The aim is to propagate knowledge and experiences about the rat EVLP model, enabling the integral understanding of the biological responses associated with that revolutionary technique.

Medicine

Monitoring Lung Function with Electrical Impedance Tomography in the Intensive Care Unit

Glasiele C. Alcala^*1, Gamonmas Ekkapat^*1,2, Kyle J. Medeiros¹, Caio C. A Morais¹, Yi Xin¹, Valentina Giammatteo^1,3, Giovanni Bruno¹, Alice Nova^1,4, Hatus Wanderley¹, Talisa Bühl¹, Marcus Victor^1,6, Timothy G. Gaulton¹, Carolyn J. La Vita⁵, Marcelo B. P. Amato⁷, Maurizio Ceradda¹, Lorenzo Berra¹, Roberta Ribeiro De Santis Santiago¹

¹Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, ²Department of Anesthesiology, Faculty of Medicine, Chulalongkorn University. King Chulalongkorn Memorial Hospital, ³Department of Anesthesiology and Intensive Care Medicine, Catholic University of Sacred Heart, ⁴School of Medicine and Surgery, University of Milano-Bicocca, ⁵Department of Respiratory Care, Massachusetts General Hospital, Harvard Medical School, ⁶Electronics Engineering, Aeronautics Institute of Technology, ⁷Instituto do Coracao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo

Electrical Impedance Tomography is a non-invasive, radiation-free, real-time pulmonary ventilation monitoring tool. By measuring impedance changes in the thorax, it can visualize the distribution of air on a breath-by-breath basis. Initially intended for ventilation monitoring, electrical impedance tomography can also measure perfusion via intravenous injection of a saline solution.