Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and MicroscopyPeter Bannas *1, Alexander Lenz *1,2, Valentin Kunick 1,2, William Fumey 1,2, Björn Rissiek 2, Joanna Schmid 1,2, Friedrich Haag 2, Axel Leingärtner 3, Martin Trepel 4, Gerhard Adam 1, Friedrich Koch-Nolte 2
1Department of Diagnostic and Interventional Radiology, University Medical Center, Hamburg, 2Institute of Immunology, University Medical Center, Hamburg, 3University Cancer Center Hamburg, University Medical Center, Hamburg, 4Department of Oncology and Hematology, University Medical Center, Hamburg
This protocol outlines the steps required to perform ex vivo validation of in vivo near-infrared fluorescence xenograft imaging experiments in mice using fluorophore labelled nanobodies and conventional antibodies.
