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University Of Massachusetts Boston

5 ARTICLES PUBLISHED IN JoVE

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Biology

PuraMatrix Encapsulation of Cancer Cells
Adnan O. Abu-Yousif 1, Imran Rizvi 1,2, Conor L. Evans 1, Jonathan P. Celli 1, Tayyaba Hasan 1,3
1Wellman Center for Photomedicine Massachusetts General Hospital, Harvard Medical School, 2Thayer School of Engineering, Dartmouth College, 3Department of Dermatology, Harvard Medical School

This video demonstrates how to encapsulate and culture cancer cells in PuraMatrix, a commercially available self assembling peptide gel.

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Bioengineering

Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models Using Particle-tracking Microrheology
Dustin P. Jones *1, William Hanna *1, Hamid El-Hamidi 1, Jonathan P. Celli 1
1Department of Physics, University of Massachusetts Boston

Particle-tracking microrheology can be used to non-destructively quantify and spatially map changes in extracellular matrix mechanical properties in 3D tumor models.

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Biology

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format
Scott M. Paulissen 1, Linda S. Huang 1
1Department of Biology, University of Massachusetts Boston

Here, sporulation of Saccharomyces cerevisiae is carried out in a 96 multiwell format.

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Developmental Biology

Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments
Liu Yang 1, Sayantanee Paul 1, Sarah DuBois-Coyne 1, Phillip Kyriakakis 2, Alexey Veraksa 1
1Biology Department, University of Massachusetts Boston, 2Department of Bioengineering, University of California, San Diego

The goal of this protocol is to provide a straightforward and inexpensive approach to collecting Drosophila embryos at medium scale (0.5-1 g) and preparing protein extracts that can be used in downstream proteomic applications, such as affinity purification-mass spectrometry (AP-MS).

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Medicine

Ultrasonography of the Adult Male Urinary Tract for Urinary Functional Testing
Teresa T. Liu *1,2, Allison C. Rodgers *3, Tristan M. Nicholson 4, Jill A. Macoska 5,6, Paul C. Marker 6,7, Chad M. Vezina 6,8, Dale E. Bjorling 6,8, Alejandro Roldan-Alzate 2,9,10, Diego Hernando 10,11, Granville L. Lloyd 12, Timothy A. Hacker 3, William A. Ricke 1,6
1Department of Urology, University of Wisconsin-Madison, 2K12 Kure, University of Wisconsin-Madison, 3Cardiovascular Research Center, Department of Medicine, University of Wisconsin-Madison, 4Department of Urology, University of Washington, 5University of Massachusetts Boston, 6U54 George M. O'Brien Center, University of Wisconsin-Madison, 7College of Pharmacy, University of Wisconsin-Madison, 8School of Veterinary Medicine, University of Wisconsin-Madison, 9Department of Mechanical Engineering, University of Wisconsin-Madison, 10Department of Radiology, University of Wisconsin-Madison, 11Department of Medical Physics, University of Wisconsin-Madison, 12Rocky Mountain Regional VA Medical Center, University of Colorado

We describe the use of high frequency ultrasound with contrast imaging as a method to measure bladder volume, bladder wall thickness, urine velocity, void volume, void duration, and urethral diameter. This strategy can be used to assess voiding dysfunction and treatment efficacy in various mouse models of lower urinary tract dysfunction (LUTD).

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