University of Wisconsin-Madison, School of Medicine and Public Health

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Trans-vivo Delayed Type Hypersensitivity Assay for Antigen Specific Regulation

Ewa Jankowska-Gan¹, Subramanya Hegde¹, William J. Burlingham¹

¹Department of Surgery, University of Wisconsin-Madison, School of Medicine and Public Health

We describe a valuable diagnostic assay that could potentially be used to decide the withdrawal of immunosuppression after transplant without elevated risk of graft rejection. The assay uses the principles of Delayed Type Hypersensitivity and provides accurate assessment of both donor specific effector and regulatory immune responses mounted by recipients.

Neuroscience

Stimulating and Analyzing Adult Neurogenesis in the Drosophila Central Brain

Kassi L. Crocker^1,2,3, Shawn Ahern-Djamali³, Grace Boekhoff-Falk^1,3

¹Genetics Graduate Training Program, University of Wisconsin-Madison, School of Medicine and Public Health, ²Science and Medicine Graduate Research Scholars Program, University of Wisconsin-Madison, School of Medicine and Public Health, ³Department of Cell and Regenerative Biology, University of Wisconsin-Madison, School of Medicine and Public Health

This article provides detailed protocols for inflicting Penetrating Traumatic Brain Injury (PTBI) to adult Drosophila and examining the resulting neurogenesis.