DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel ElectrophoresisEliza L. Lewis 1, Aaron M. Leconte 1
1Department of Chemistry, W.M. Keck Science Department of Claremont Mckenna, Pitzer, and Scripps College
This protocol describes the characterization of DNA polymerase synthesis of modified DNA through observation of changes to near-infrared fluorescently labeled DNA using gel electrophoresis and gel imaging. Acrylamide gels are used for high resolution imaging of the separation of short nucleic acids, which migrate at different rates depending on size.
