Worcester Polytechnic Institute- WPI

2 ARTICLES PUBLISHED IN JoVE

Biology

Heterokaryon Technique for Analysis of Cell Type-specific Localization

Roseann Gammal^*1, Krista Baker^*1, Destin Heilman¹

¹Department of Chemistry and Biochemistry, Worcester Polytechnic Institute- WPI

A flexible and efficient method for the characterization of cell type-specific protein localization and nucleocytoplasmic shuttling is described. This heterokaryon approach uses fluorescently-labeled fusion proteins to image protein localizations after cell fusion. The protocol is amenable to steady-state localizations or more dynamic determinations based on live cell imaging.

Biology

Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens

Yen-Chun Liu¹, Luis Vidali¹

¹Department of Biology and Biotechnology, Worcester Polytechnic Institute- WPI

A simple and efficient method to transform Physcomitrella pantens protoplasts is described. This method is adapted from protocols for Physocmitrella protonemal protoplast and Arabidopsis mesophyll protoplast transformation¹.