Preparing Paraffin Sections of Kidney and Peyer's Patch Isolated from an IgAN Murine Model for Histological and Immunohistochemical Analyses

To begin preparing the paraffin sections of the kidney and Peyer's patch isolated from properly-anesthetized IgA neuropathy model mouse, first fix one Peyer's patch or three-millimeter-thick kidney tissues with 4%paraformaldehyde for 24 hours. Then dehydrate them using a gradient of ethanol and xylene before washing them with water. Submerge the tissues in wax for two hours before sealing and freezing them.

Next, cut four-micron-thick Peyer's patch sections or two-micron-thick kidney sections and place them in warm water to unfold. Using a clean glass slide, gently pick up the unfolded slices and incubate them in a constant temperature oven at 40 degrees Celsius for one hour before staining.