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Image Analysis in Gen5 Software to Analyze Drug Response in Patient-Derived Organoids Using Multiplexed Live-Cell Imaging

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72 Views

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02:40 min

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January 5th, 2024

DOI :

10.3791/201046-v

January 5th, 2024

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Kaitriana E. Colling*¹^,², Emily L. Symons*¹, Lorenzo Buroni*³, Hiruni K. Sumanasiri¹, Jessica Andrew-Udoh¹, Emily Witt¹^,⁴, Haley A. Losh¹, Abigail M. Morrison¹, Kimberly K. Leslie⁵, Christopher J. Dunnill⁶, Johann S. de Bono³, Kristina W. Thiel¹^,⁷

¹Department of Obstetrics and Gynecology, Carver College of Medicine, University of Iowa, ²Cancer Biology Graduate Program, Carver College of Medicine, University of Iowa, ³The Institute of Cancer Research: and the Royal Marsden NHS Foundation Trust, ⁴Department of Radiation Oncology, Carver College of Medicine, University of Iowa, ⁵Division of Molecular Medicine, Departments of Internal Medicine and Obstetrics and Gynecology, University of New Mexico Comprehensive Cancer Center, University of New Mexico Health Sciences Center, ⁶Agilent Technologies, ⁷Holden Comprehensive Cancer Center, University of Iowa

* These authors contributed equally

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Keywords Image Analysis

Patient derived Organoids

Multiplexed Live cell Imaging

Cellular Analysis

Object Selection

Subpopulation Analysis

Cellular Debris

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