Sample Preparation for Imaging Early Zebrafish Embryos Within an Intact Chorion Using a Light-Sheet Microscope

To begin, remove the prepared agarose tube with added beads from a 37 degree Celsius water bath. Pour the entire 10 milliliters of agarose onto a six centimeter Petri dish. Once the temperature drops below 33 degrees Celsius, transfer 10 to 15 emb