Preparation of Bacterial ( N. meningitidis) Suspension
3:09
Seeding and Infection of Target Cells
4:29
Immuno-staining
6:21
Confocal Laser Scanning Microscopy
8:56
Quantitative Colocalization Analysis
9:55
Intracellular Localization of Opc-expressing N. meningitidis and Alpha-actinin
12:23
Conclusion
Neisseria meningitidis (Nm), a gram negative human-specific respiratory pathogen, can bind to human α-actinin. Here we present a protocol for visualisation of colocalisation of the bacterium with intracellular α-actinin after bacterial entry into human brain microvascular endothelial cells (HBMECs).