Concentration and Subretinal Injection of Plasmid DNA
3:46
Electroporation
3:46
Neonatal Mouse Retinas Electroporated with pCAG-EGFP Over Developmental Time
5:15
Conclusion
A method for the incorporation of plasmid DNA into murine retinal cells for the purpose of performing either gain- or loss of function studies in vivo is presented. This method capitalizes on the transient increase in permeability of cell plasma membranes induced by the application of an external electrical field.