Preparation of Fertilized Zebrafish Embryos and Injection with Morpholinos
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Phenotype Rescue Experiments
6:42
Florescent Imaging of Injected Embryos
7:55
Results: Analysis of wnt5a Morpholino Knockdown in Zebrafish
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Conclusion
We describe a method of generating a possible zebrafish model of polycystic kidney disease. We used Tg(wt1b:GFP) fish to visualize kidney structure. Knockdown of wnt5a was by morpholino injection. Pronephric cyst formation after wnt5a knockdown was observed in this GFP transgenic zebrafish.