Seeding and Culturing of HL-1 Atrial Myocytes on Coverslips
4:12
Loading HL-1 Cells with Calcium- and Voltage-sensitive Dyes
5:20
Dual Channel Optical Mapping of HL-1 Cells
6:42
Results: Action Potentials and Calcium Signals Propagate Across a Monolayer of HL-1 Atrial Myocytes
7:41
Conclusion
This article describes the technique used to perform dual channel optical mapping in cultured HL-1 atrial cell monolayers. This unique protocol allows the simultaneous visualization of both calcium (Ca) and voltage (Vm) activity in the same area for the detailed detection and analysis of electrophysiological properties of culture monolayers.