Dissection of Embryos, Tissue Dissociation, and Cell Plating
6:32
3D Neuronal Network Construction
7:28
Results: Examples of 3D Networks Captured by Confocal Microscopy and by Electrophysiological Recordings
8:24
Conclusion
In this work, a novel experimental model in which 3D neuronal cultures are coupled to planar Micro-Electrode Arrays (MEAs) is presented. 3D networks are built by seeding neurons in a scaffold made up of glass microbeads on which neurons grow and form interconnected 3D structures.