Fibroblast Culture and Plating for Epigenetic Conversion
4:00
Increase Cell Plasticity Using the De-methylating Agent 5-aza-CR
5:31
Pancreatic Differentiation Protocol
6:47
Results: Morphological and Functional Changes During Epigenetic Conversion
8:02
Conclusion
TRANSCRIÇÃO
The overall goal of this procedure is to convert fibroblasts into insulin-secreting cells without inducing pluripotency or using retrovital vectors. This method can help answer key questions in the regenerative medicine field and in particular in
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Here, a new method that allows the conversion of adult skin fibroblasts into insulin-secreting cells is presented. This technique is based on epigenetic conversion, does not involve the use of retroviral vectors nor the acquisition of a stable pluripotent state. It is therefore highly promising for translational medicine applications.