July 11th, 2016
•We present a rapid and flexible protocol for a single T cell receptor (TCR) retroviral-based in vivo expression system. Retroviral vectors are used to transduce bone marrow progenitor cells to study T cell development and function of a single TCR in vivo as an alternative to TCR transgenic mice.
Tags
Vídeos Relacionados
Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
Retroviral Transduction of T-cell Receptors in Mouse T-cells
Bone Marrow-derived Macrophage Production
Systemic Injection of Neural Stem/Progenitor Cells in Mice with Chronic EAE
Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation
Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function
An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells
Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells
Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados