Preparation of Cellular Lysate from Primary Adipocytes
7:00
Binding and Elution of RBP
8:45
Results: RNA Pull-down Assay
10:05
Conclusion
Transcrição
The overall goal of this experiment is to retrieve RNA-binding proteins of an RNA transcript from brown adipose tissue. This method can help answer key questions in this field, such as, what are the regulatory mechanisms of longlife coding RNA, an
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An RNA pull-down protocol is optimized here for detection of interactions between RNA-binding proteins (RBPs) and noncoding as well as coding RNAs. An RNA fragment from androgen receptor (AR) was used as an example to demonstrate how to retrieve its RBP from lystate of primary brown adipocytes.