The overall goal of this procedure is to image the heart function of Drosophila longitudinally in vivo using an optical coherence microscopy technique. This method can help answer key questions in the field of Drosophila cardiac structural and fun
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Here, the experimental protocols are described for preparing Drosophila at different developmental stages and performing longitudinal optical imaging of Drosophila heartbeats using a custom optical coherence microscopy (OCM) system. The cardiac morphological and dynamical changes can be quantitatively characterized by analyzing the heart structural and functional parameters from OCM images.