The overall goal of this experiment is to assess the effectiveness of insecticides in killing bed bug eggs and first instars. This method can help answer key questions in the entomology field, such as the effects of toxicological assays on bed bug
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Insecticide evaluations are often targeted against adult insects, rather than immature stages. Here, we present a protocol for evaluating insecticides against bed bug eggs with a comparison to the first nymphal bed bug stage. These protocols could be adjusted for other insects to evaluate insecticide efficacy in non-adult life stages.