Culture and Expansion of Cytokine Transduced Stromal Cells
3:05
Phospho-flow Cytometry Analysis of Stroma-produced Cytokine Activity
5:09
Injection of Cytokine-transduced Stroma into Mice
6:22
Serial Blood Collection and Plasma Monitoring of Exogenous Human Cytokine in Mice
8:33
Human Hematopoietic Cell Transplantation
9:53
Results: Achieving Physiological Levels of the Human TSLP Cytokine in PDX Mice
11:22
Conclusion
Transcrição
The overall goal of this procedure is to produce xenograft mice with circulating human cytokines. This method can help answer key questions in the cancer and hematopoiesis fields such as, What is the role of a specific cytokine in normal and malig
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Described here is a method for producing exogenous cytokine in patient-derived xenograft (PDX) mice via weekly intraperitoneal injection of a cytokine-transduced stromal cell line. This method broadens the utility of PDX and provides the option for transient or sustained exogenous cytokine delivery in a multitude of PDX models.