In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

0:37

LEW.1W to LEW.1A Allograft Procedure

2:18

Isolation of Allograft APCs (pDCs)

6:12

Isolation of Responder Effector T Cells and Regulatory T Cells (CD4⁺ and CD8⁺CD45RC^low)

10:30

CFSE Labeling of Responder Cells to Measure their Proliferation

12:09

FACS Staining and Analysis After 6 Days of Coculture

13:49

In Vivo Assessment of the Cells Supressive Activity by Adoptive Cell Transfer in a Heart Grafted Recipient

16:33

Representative Results

18:03

Conclusion