Obtaining Mouse Brain Synaptosomes Using a Discontinuous Sucrose Gradient
4:37
Pre-post and Extra-synaptic Fraction Isolation
6:52
Analyze Samples and Validate Fractionation
7:26
Representative Results
8:53
Conclusion
Transcrição
The overall goal of this procedure is to isolate proteins belonging to different synaptic compartments from mice brain. Describing the distribution of kinetic transmissioncomponents is essential to understand synaptic function both in normal and p
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Here, we present a brain membrane fractionation protocol that represents a robust procedure to isolate proteins belonging to different synaptic compartments.