Dissection of the Gut Epithelium from R. microplus
1:56
Epithelial Cell Dissociation
3:36
Isolation of Single Epithelial Cells Using a Percoll Gradient
6:47
Cell Surface Biotinylation and the Isolation of Biotinylated Surface Proteins
9:44
Representative Results
11:17
Conclusion
Transcrição
Purification of biotinylated cell surface protein from Rhipicephalus microplus epithelial gut cells. Within this study, a modified Percoll gradient-based methodology was utilized to isolate individual epithelial cells from Rhipicephalus microplus
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A modified density centrifugation gradient-based methodology was utilized to isolate epithelial cells from Rhipicephalus microplus gut tissue. Surface-bound proteins were biotinylated and purified through streptavidin magnetic beads for utilization in downstream applications.