Stimulation and Measuring OR Activity Using the Real-time cAMP Assay
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Results: Kinetic Measurements of the Response of OR5AN1 to Muscone
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Conclusion
Transcrição
The overall goal of this real-time high-throughput cyclic AMP assay is to screen odorants against odorant receptors and vice versa. Since mice are useful for investigating odorant receptor functioning in that it allows for larger scale screenings
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Characterizing the function of odorant receptors serves an indispensable part in the deorphanization process. We describe a method to measure the activation of odorant receptors in real time using a cAMP assay.