Uniform 15N-labeled Protein Expression in BL21 ΔE3 E. coli
5:24
Purification of Recombinant Protein from E. coli
7:50
Chemical Attachment of Glucose-5-MTS to Protein by Dialysis
8:48
Results: Site-selective Cys Mutation Followed by In Vitro Glycosylation in STIM1 EF-SAM Protein
10:11
Conclusion
Transcrição
The overall goal of this procedure is to incorporate cysteine residues in proteins by site directed mutagenesis at natively glycosylated sites and employ in-vitro glucose attachment and assessment approaches to evaluate the efficiency and structur
Faça login ou inicie seu teste gratuito para acessar este conteúdo
Biochemical and structural analyses of glycosylated proteins require relatively large amounts of homogeneous samples. Here, we present an efficient chemical method for site-specific glycosylation of recombinant proteins purified from bacteria by targeting reactive Cys thiols.