Preparation of NGM Plates Containing Either 1% DMSO or 750 μM Etoposide
3:44
Measurement of C. elegans Growth
5:26
C. elegans Egg Laying Assay
6:18
Results: C. elegans Growth and Reproduction with Etoposide Treatment
7:41
Conclusion
Transcrição
The overall goal of this procedure is to measure the effect of chemicals, such as the topoisomerase inhibitor etoposide, on the growth and reproduction of the model animal Caenorhabditis elegans. This method can help answer key questions in the to
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A basic protocol to evaluate the toxicity of chemicals in a model animal, Caenorhabditis elegans, is described. The method is convenient and useful for the development of pharmaceuticals as well as for the risk assessment of various environmental pollutants.