Visualize Uptake of Extracellular Vesicles by Confocal Microscopy
6:18
Endothelial Cell Permeability
7:36
Determine Puromycin Sensitivity
8:59
Transduction of Endothelial Cells with Lentiviral Vector
10:53
Results: Use of Confocal Microscopy and Diffusion Capacity of Fluorochrome Dextran to Study the Interaction Between the Extracellular Vesicles and Endothelial Cells
12:50
Conclusion
Transcrição
The overall goal of this procedure is to investigate the function of extracellular vesicles released by the Malaria parasite infected red blood cells. This method can help answer key questions in the Malaria fields, such as how the parasites regul
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In this work, we describe protocols to investigate the role of extracellular vesicles (EVs) released by Plasmodium falciparum infected erythrocytes. In particular, we focus on the interactions of EVs with endothelial cells.