Evaluation of Capture Yield by qPCR with Primers for the Target Capture Region of Interest
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Results: Hybridization Capture of Chromatin-associated Proteins in Mammalian Cells
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Conclusion
Transcrição
This method can help answer key questions in our understanding of genome regulation, such as how disease associated sequence variants in non-coding regions affect gene expression and regulation by altering protein binding, and it can help identify
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This is a method to identify novel DNA-interacting proteins at specific target loci, relying on sequence-specific capture of crosslinked chromatin for subsequent proteomic analyses. No prior knowledge about potential binding proteins, nor cell modifications are required. Initially developed for yeast, the technology has now been adapted for mammalian cells.